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KMID : 0942820100090010003
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Journal of Korean Brain Tumor Society
2010 Volume.9 No. 1 p.3 ~ p.7
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Detecting Loss of Heterozygosity at 1p and 19q in Anaplastic Oligodendroglioma Patients
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Lee Chae-Heuck
Joo Mee
Kang Yun-Kyung
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Abstract
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Objective: Combined loss of heterozygocity(LOH) on 1p and 19q is reported in 50% to 90% of oligodendrogliomas
and has proved a favorable prognostic factors especially in sensitivity on chemotherapy. Flourescence in situ hybridization(FISH) and polymerase chain reaction(PCR) are the most widely used techniques to detect 1p and/or 19q loss. The aim of this study was to find the 1p and/or 19q loss of personal series of anaplastic oligodendrogliomas.
Materials and Methods: The author retrospectively analyzed the available paraffin blocks of resected tumors of 28
anaplastic oligodendroglioma patients admitted to the hospital between January 2000 and August 2007. Tissue microarray
and FISH analysis were done by the pathology of Seoul National University Hospital.
Results: FISH result was encountered in 17.9%(5/28 cases) for 1p loss, 14.3%(4/28 cases) for 19q loss, 10.7%(3/28cases) for 1p and 19q loss. Primary anaplastic oligodendroglioma patients had 1p and/or 19q loss in 22.7%(5/22 cases), however recurrent anaplastic oligodendroglioma patients in 33.3%(2/6 cases). This result was not statistically significant( p value>0.05).
Conclusion: FISH study to detect 1p and/or 19q loss in personal 28 cases was unexpectedly low, and this result might be extreme normal variation. However, I cannot exclude unknown errors of research such as inadequate pathologic tissues from old paraffin blocks. I would like to further study to compare the PCR result. I think that it is necessarily to detect 1p and/or 19q loss in touch preparation of fresh tumor tissues.
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KEYWORD
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Loss of heterozygocity(LOH), Flourescence in situ hybridization(FISH), Polymerase chain reaction(PCR), Anaplastic oligodendroglioma
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